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To your handle wells, MSM is added to the new wells up until a maximum of 2 hundred ?l is achieved. To set up for every assay, 96 better plates were filled with crude oil which was complexed with Nile Yellow (ten ul off Nile Red per ml off rough petroleum). To determine the amount of rough petroleum degraded by germs, we used the Nile Purple assay method discussed within the French and you can Terry7. OD indication were taken on a plate reader during the 600 nm in the four time factors (D0, D3, D5, and D10).
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